Efeito de sacarose e giberelina no crescimento in vitro do tomate de árvore (Cyphomandra betacea)

Abstract

The method of propagating plants in tissue culture is often indicated for mass multiplication for various plant species. Among these stands Tree Tomato (C. betacea) where its fruits have significant commercial value in some regions of the world. To make its propagation to larger scale levels, studies are needed to optimize the supplementation of the culture medium and ensure its propagation in vitro. In this sense, the objective of this study was to evaluate the results of different sucrose levels such as: 0; 10; 20; 30; 40 and 50 g L-1 in the first test, after identification of the best dosage, it was used in the second test to evaluate different levels of GA3 (gibberellic acid) such as 0; 0.25; 0.5; 1.0 and 2.0 mg L -1, both tests inoculated in MS culture medium for 45 days. The tests were conducted at the Plant Tissue Culture laboratory of IFMG Campus Bambuí. The explants used for both assays were from mother plants already established in vitro. The experimental design was completely randomized, with 5 treatments, 6 repetitions and, each repetition consisting of 4 tubes. Sucrose assay data were subjected to analysis of variance and polynomial regression (p <0.05), and treatment means for the GA3 assay were statistically compared by the Scott Knott test (p <0.05), both tests using the SISVAR software. The variables analyzed for both trials were: number of shoots, length of largest shoot, number of nodes, seedling height, number of leaves, presence and absence of roots, presence and absence of corns and dry weight. It was concluded that the dosage of 30 g L-1 sucrose had a better influence on shoot length, number of nodes, seedling height, number of leaves and callus, the other variables did not interfere with sucrose levels. . It was verified that, the different dosages of GA3 tested, did not influence any of the analyzed variables.