Caracterização das comunidades procarioticas do Queijo Minas Artesanal Canastra e sua importância para sustentabilidade
Data
Autor(es)
Orientado(es)
Título da Revista
ISSN da Revista
Título de Volume
Editor
Abstract
Canastra Artesanal Minas Cheese is the most important typical product of Serra da Canastra region, being the main income source of the region's population. Its physical and sensorial aspects are due to the conditions of the region in which it is produced and the way of preparation, which promote a selection of microorganisms capable of conferring the characteristics that add value to the product. Thus, it is essential to have full knowledge of this product microbial composition, as well as its dynamics throughout the production process, in order to evaluate which group acts in which phase of the process, where they appear and which are beneficial and harmful to the composition of the final product. Therefore, the present work has proposed to characterize the bacterial communities present throughout the production process of the Canastra Artesanal Minas Cheese and between different producers. For such purpose the technique of metagenomics, a technique of culture independent identification through which it is possible to obtain thousands of DNA sequences and characterization of any bacterial community present in a sample, was used. The study of these groups dynamics throughout the production process allowed to identify that the microbiota present in the final product is more similar to “pingo” (meaning drop) microbiota, highlighting the importance of the same in the production process. The composition analysis of the communities indicated that possibly the microorganisms that generate the characteristics that disaggregate value to the products are in the sources that constitute the utensils and work facilities of the producers. The genus Staphylococcus, which harbors pathogenic species, showed a relative frequency loss during the maturation process on high value-added farms and an increase in the frequency of low value-added farms, which requires an alert since bacteria of this genus can lead to food poisoning. The knowledge of the microbiota associated to the production process of this type of cheese allows to identify the ports of entry of microorganisms harmful to the process, thus allowing a greater control of these microorganisms and consequently increase the added value of the final product, a factor that can guarantee the sustainability of this important local productive process for the Serra da Canastra region.
